EPSILOMETER TEST PDF

Martin, M. Nouvellon, M. Pestel, J. Pons, J. The antimicrobial susceptibilities of clinical isolates of coagulase-negative staphylococci to teicoplanin were determined by disc diffusion and the Epsilometer test E test and the results were compared with the MICs determined by the agar dilution method of the National Committee for Clinical Laboratory Standards NCCLS.

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The purpose of the study was to validate the Epsilometer test E-test method for antimicrobial susceptibility testing of selected periodontopathic microorganisms using the agar dilution method as a standard. The E-test has been developed to provide a direct quantification of antimicrobial susceptibility of microorganisms.

The device consists of a predefined, continuous, and exponential gradient of antibiotic concentrations immobilized along a rectangular plastic test strip.

After 48 hours incubation a drop-shaped inhibition zone intersects the graded test strip at the inhibitory concentration IC of the antibiotic. A total of 60 strains of key periodontal pathogens Prevotella intermedia, Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans, Eikenella corrodens, Campylobacter rectus, and Capnocytophaga isolated from 22 sites of 12 patients were studied.

The antibiotics tested were clindamycin, metronidazole, tetracycline, ciprofloxacin, cefoxitin, and ampicillin at concentrations above and below the achieved blood or gingival crevicular fluid levels. As a standard reference the minimal inhibitory concentrations MICs were determined using the agar dilution method. The E-test ICs for ampicillin, cefoxitin, and metronidazole against the Gram-negative capnophilic and microaerophilic rods and the black-pigmented anaerobic rods ICs for ampicillin, clindamycin, metronidazole, and tetracycline showed a high percentage of agreement with the agar dilution MICS.

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Save Cancel. Create a file for external citation management software Create file Cancel. Full-text links Cite Favorites. Abstract The purpose of the study was to validate the Epsilometer test E-test method for antimicrobial susceptibility testing of selected periodontopathic microorganisms using the agar dilution method as a standard.

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Madinier IM, et al. Med Pregl. PMID: Review. Proteolytic and hydrolytic enzymes from putative periodontal pathogens: characterization, molecular genetics, effects on host defenses and tissues and detection in gingival crevice fluid.

Eley BM, et al. Periodontol No abstract available. Show more similar articles See all similar articles. Patil N, Mule P. Patil N, et al. Infect Drug Resist. Park CS, et al. Animals Basel. Punjabi K, et al. Front Microbiol. Repositioning of 8-hydroxyquinoline derivatives as a new promising candidate for combating multidrug resistant Neisseria gonorrhoeae. Lawung R, et al.

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Clin Oral Investig. Epub Jul 2. Show more "Cited by" articles See all "Cited by" articles. MeSH terms Adult Actions. Aged Actions. Female Actions. Humans Actions.

Male Actions. Middle Aged Actions. Substances Anti-Bacterial Agents Actions. Metronidazole Actions. Clindamycin Actions. Ciprofloxacin Actions. Cefoxitin Actions. Ampicillin Actions.

Tetracycline Actions. Full-text links [x] Wiley. Copy Download.

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E-TEST ( Epsilometer test): Principle, purpose, procedure, results and interpretations

Last Updated on: February 5, by Sagar Aryal. It is a cost-effective tool that has been developed to provide a direct quantification of antimicrobial susceptibility of microorganisms. It is a quantitative method that applies both the dilution of antibiotics and the diffusion of antibiotics into the medium. More precisely, the test combines a disk diffusion pattern with the determination of Minimum Inhibitory Concentration MIC which is considered as the lowest concentration of an antimicrobial that inhibits the visible growth of a microorganism. The E-test uses rectangular plastic strips with the predefined, continuous and exponential gradient of antibiotic concentration one side of the strip contains the antimicrobial agent concentration gradient and the other side of the strip contains a numeric scale that indicates the drug concentration. The e test strip is then placed on to an inoculated agar plate.

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Epsilometer test (E test)

E Test: a novel technique for antimicrobial susceptibility testing. We describe the applicability of the E test AB Biodisk Solna, Sweden , a new method for determining minimum inhibitory concentrations of antimicrobial agents against bacteria. This report is based on the literature review and on our own experience using the E test for susceptibility testing of the Xanthomonas maltophilia, Streptococcus pneumoniae and Streptococcus viridans group against eight different drugs. Measurement of the susceptibility of microorgan isms to antimicrobial agents is of great impor tance in the rational use of antimicrobial agents, in the evaluation of new drugs, and in epidemiological studies. However, the results of such measurements are not an absolute value because they are influenced sometimes markedly, by the test conditions used. Differences in each factor such as inoculum time, may all affect the amount of antimicrobial agent required to inhibit the organism in vitro 7.

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E-test: A New Technique for Antimicrobial Susceptibility Testing for Periodontal Microorganisms

The E-test has been developed to provide a direct quantification of antimicrobial susceptibility of microorganisms. E-test is a laboratory test used to determine minimum inhibitory concentration MIC and whether or not a specific strain of bacterium or fungus is susceptible to the action of a specific antibiotic. E test strip is a non-porous plastic strip immobilized with predefined continuous and stable gradient of 15 antibiotic concentrations on one side and printed with an MIC scale on the other side of nylon strips. E-test has an extensive range of over antimicrobial references that can be classed into 4 categories:. E test is a quantitative technique that is based on combination of concept of both dilution and diffusion principle for susceptibility testing. E test strip is placed on to an inoculated agar plate; there is an immediate release of antibiotics from the plastic carrier surface into the agar surface. After incubation, bacterial growth becomes visible, symmetrical inhibition ellipse along the strip is seen.

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The antimicrobial susceptibilities of clinical isolates of Pseudomonas aeruginosa to six antipseudomonal antibiotics were tested by five methods: the National Committee for Clinical Laboratory Standards NCCLS methods for broth microdilution, agar dilution, and agar disk diffusion; the Vitek Automicrobic System method Vitek Systems, Hazelwood, Mo. Some discrepancies were observed between results from the E test and broth methods for gentamicin, with the broth microdilution and Vitek methods giving higher MICs than the E test and other methods using agar. The most recent NCCLS guidelines for broth dilution testing have reduced the recommended levels of cation supplementation, which may enhance future agreement between results for the aminoglycosides and P. We found that the E test offers a simple, labor-efficient, and accurate method for MIC determination on an agar medium. National Center for Biotechnology Information , U. Journal List J Clin Microbiol v.

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